superscript preamplification system for first-strand cdna synthesis Search Results


oligo dt 12 18 primer  (Thermo Fisher)
99
Thermo Fisher oligo dt 12 18 primer
Oligo Dt 12 18 Primer, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/superscript+preamplification+system+for+first-strand+cdna+synthesis/Oligomycin/pmc04624333-100-42-53
Average 99 stars, based on 1 article reviews
oligo dt 12 18 primer - by Bioz Stars, 2026-10
99/100 stars
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strand complementary dna cdna synthesis kit  (Thermo Fisher)
99
Thermo Fisher strand complementary dna cdna synthesis kit
FIG. 4. SSTR mRNA expression in TT cells. Extracted RNA (1 mg/reaction) was treated with deoxyribonuclease and subjected to RT. Aliquots from the generated <t>cDNA</t> were subjected to sub- sequent PCR amplification of SSTRs, using the primers indicated in Table 1. PCR products were resolved on a 2% agarose gel. The expected PCR products of SSTR1–5 are indicated with arrows (lane M, PCR marker; lane G, PCR product of GAPDH amplification).
Strand Complementary Dna Cdna Synthesis Kit, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/superscript+preamplification+system+for+first-strand+cdna+synthesis/DNA/pm11344221-65-3-17
Average 99 stars, based on 1 article reviews
strand complementary dna cdna synthesis kit - by Bioz Stars, 2026-10
99/100 stars
  Buy from Supplier

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FIG. 4. SSTR mRNA expression in TT cells. Extracted RNA (1 mg/reaction) was treated with deoxyribonuclease and subjected to RT. Aliquots from the generated cDNA were subjected to sub- sequent PCR amplification of SSTRs, using the primers indicated in Table 1. PCR products were resolved on a 2% agarose gel. The expected PCR products of SSTR1–5 are indicated with arrows (lane M, PCR marker; lane G, PCR product of GAPDH amplification).

Journal: The Journal of clinical endocrinology and metabolism

Article Title: Somatostatin receptor subtypes 2 and 5 differentially affect proliferation in vitro of the human medullary thyroid carcinoma cell line tt.

doi: 10.1210/jcem.86.5.7489

Figure Lengend Snippet: FIG. 4. SSTR mRNA expression in TT cells. Extracted RNA (1 mg/reaction) was treated with deoxyribonuclease and subjected to RT. Aliquots from the generated cDNA were subjected to sub- sequent PCR amplification of SSTRs, using the primers indicated in Table 1. PCR products were resolved on a 2% agarose gel. The expected PCR products of SSTR1–5 are indicated with arrows (lane M, PCR marker; lane G, PCR product of GAPDH amplification).

Article Snippet: Using a first strand complementary DNA (cDNA) synthesis kit (SuperScript Preamplification System for First Strand cDNA Synthesis; Life Technologies, Inc.), 1 mg total RNA was reverse transcribed with random hexamers according to the manufacturer’s protocol.

Techniques: Expressing, Generated, Amplification, Agarose Gel Electrophoresis, Marker